pET-32 Xa/LIC pET32 Xa/LIC载体质粒图谱、序列、价格、抗性、测序引物、大小等基本信息

出品公司:	EMD Biosciences(Novagen)
别名:	pET32 Xa/LIC, pet 32 Xa/LIC
质粒类型:	大肠杆菌蛋白表达
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5926 bp
5' 测序引物:	T7或者Trx-F
5' 测序引物序列:	T7: 5'-TAATACGACTCACTATAGGG-3'; Trx-F: 5' TTCCTCGACGCTAACCTG 3'
载体标签:	thioredoxin (N端); His (中间和C端), N- Factor Xa
载体抗性:	Ampicillin
表达宿主菌:	Origami2(DE3) 、 Origami(DE3)、OrigamiB(DE3)、BL21-TrxB(DE3)
备注:	For directional cloning of PCR-amplified DNA; ligation independent cloning; Factor Xa cleavage site
产品目录号:	69072-3
稳定性:	瞬时表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pet32 Xa/LIC载体图谱

pet32 Xa/LIC克隆方法

Feature Name	Start	End
T7_terminator	129	1
T7_Terminal_primer	69	87
S15	319	275
lacI	328	2279
6xHIS	370	353
T7_transl_en_RBS	743	727
lacO	788	761
T7_promoter	806	788
tet (300 - 563)	842	1105
pBRrevBam_primer	913	894
tet (576 - 851)	2338	2613
ROP	3088	3279
pGEX_3_primer	3295	3273
pBR322_origin	4313	3694
Ampicillin	5328	4468
AmpR_promoter	5398	5370

ORF	Start	End
ORF frame 1	718	137
ORF frame 2	353	1447
ORF frame 3	1320	2279
ORF frame 2	5328	4468

Enzyme Name	Cut
XhoI	158
NotI	166
EagI	166
HindIII	173
SalI	179
SacI	190
EcoRI	192
BamHI	198
EcoRV	206
NcoI	211
AgeI	257
KpnI	264
BglII	267
BstBI	294
XbaI	755
BclI	1561
ApaI	1758
HpaI	2053
PstI	4786

The pET-32 Xa/LIC vector is designed for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with the 109aa Trx•Tag™ thioredoxin protein (1), His•Tag® and S•Tag™ sequences. Using specifically designed primers for amplification and the pET-32 Xa/LIC Vector Kit (Cat. No. 69072-3), inserts can be efficiently cloned without the need for restriction digestion or ligation, and the resulting fusion proteins can be cleaved with Factor Xa to precisely remove all vector-encoded amino acids. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

LOCUS       pET-32 Xa/LIC   5926 bp    DNA Circular SYN
DEFINITION pET-32 Xa/LIC
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="pET-32 Xa/LIC"
                     /mol_type="other DNA"
     terminator      complement(1..129)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     CDS             complement(137..718)
                     /label="ORF frame 1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
                     GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
     misc_feature    complement(275..319)
                     /label="S15"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
                     GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
     misc_feature    328..2279
                     /label="lacI"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
                     GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
     CDS             353..1447
                     /label="ORF frame 2"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(353..370)
                     /label="6xHIS"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(727..743)
                     /label="T7_transl_en_RBS"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(761..788)
                     /label="lacO"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     promoter        complement(788..806)
                     /label="T7_promoter"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     gene            842..1105
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(894..913)
                     /label="pBRrevBam_primer"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     CDS             1320..2279
                     /label="ORF frame 3"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     gene            2338..2613
                     /label="tet (576 - 851)"
                     /gene="tet (576 - 851)"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    3088..3279
                     /label="ROP"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    complement(3273..3295)
                     /label="pGEX_3_primer"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     rep_origin      complement(3694..4313)
                     /label="pBR322_origin"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     gene            complement(4468..5328)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(4468..5328)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5370..5398)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct
241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt
301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat
361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca
421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca
481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt
541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg
601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg
661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat
721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta
841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa
1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga
1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc
2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg
2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg
2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca
2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc
2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca
3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac
3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg
3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg
3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc
3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg
3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca
//

pHDE-35S-Cas9-mCherry-UBQ	pET-28a(+)
pGEX-6P-3	pUCD800
pSI	pMET B
pAAV-RC9	pSup2021
pGL4.24[luc2P/minP]	pFC-MEKK
pLVX-IRES-tdTomato	pJP4
pCMV-Myc-C	pcDNA3.1-mRFP
pL-brevisGH1-MBP	p3
pCold III	pMKO.1-puro
pET-3a(+)	pET-24b(+)
pTRKH3-ldhGFP	pIEXBac-c-EGFP-1
pFLAG CMV 5.1	pCMV-Tag 3C
pCMV-SPORT6.1	pCR3-9
pLH3	pPIC9K
pBI101 T-DNA	pC221
pSP64-fl+	pMembraneprotein_TA
pET-HY3	pOPI3CAT
unnamed plasmids	pBARGEM7-1
pCMV-Tag3	pcDNA3.1(+)
pRetroX-TetOne-luc	pLB1
Super PiggyBac Transposase	pGreenZeo lenti reporter
pET-21c(+)	pHV1249
pEE12.4	pCoHygro
pRU668	pGL4.28[luc2CP/minP/Hygro]
pEXT-20	pEF4/myc-His B
pGEX-5X-1	pTRE2hyg
pENTR-L5-pLac-Spec-L2	pBL1
pGL4.43[luc2P/XRE/Hygro]	pbRF_TA
pRS315	pPermease_TA
pCMV-Gaussia-Dura-Luc	pCDH-EF1-MCS-(PGK-Puro)
pHIC-PI	pABC-ATP_TA
pGL4.34[luc2P/SRF-RE/Hygro]	pLP1
pTALETF_v2 (HD)	pAAV-RC
pcDNA3.2/V5-DEST	pEXP-Lib
pacAd5 9.2-100	pCAGGS
pGEX-TEV-Bgl3082(3)	pENTR5-UbCp
pDC511	pMKS-1
pNTAP-Shuttle-C	pDONR222
pTrcHis2 B	pET-32 Xa/LIC
pDNApolymeraseIIIGammaTau_TA	pAAV-UbC
pYM39-EYFP	pGEX-5X-2
pYC2/NT C	pRK793
PinPoint Xa-3	pMSCVneo
pET302/NT-His	pBad/gIII A
pGL2-Basic	pBD10
pFB	pPhosphoglyceroltransferase_TA
p53-Luc	pIJ702
pCRII-TOPO/25-6	pCDH-ECMV-MCS-3×FLAG-EF1α-ZsGreen1-T2A
pGL4.71[hRlucP]	pEarleyGate203
pMCS-Green-Renilla-Luc	pET-48b(+)
SARS Frag. 8/yT&A	p3XFLAG-CMV-7.1
pHisSi-1	pUTK9
pGL4.30	YIP33
pNO1517	pEF1α-IRES-ZsGreen1
pcDNA3.1/NT-GFP-TOPO	pTT5
pcDNA4/HisMax A	pAD123
pGEX-KG	pSUPERIOR.puro
pGEMEX-1	pGEX-5X-3
pRecF_TA	pJLO
pVitro2-neo-mcs	pET-17b
pQE-1	pMMB66EH
pIRES2-EGFP	pLVX-EF1α-DsRed-Monomer-N1
pGL4.25[luc2CP/minP]	pcambia35s-ECFP
pZsGreen1-1	pPIC6 C
pHE3	pET151/D-TOPO
pMT-Bip-V5-HisA	pBMTB-3
pSEAP2-Basic	pXINSECT-DEST39
pRU671	SARS Frag. 4 pCRII-TOPO
pSico	pSIH1-H1-CopGFP
pSP64	pPZP202
pB18	pBC-Hygro
pMUTIN-HA	pBI-CMV2
pLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro	pLenti6.3-MCS-IRES2-EGFP
pEF6/V5-His TOPO TA	pCAMBIA5105
pCMV-3Tag-2A	RM53
pLVX-Puro	pcDNA3.1+N-HA
pHcRed1-DR	pcambia35s-EGFP
pCAMBIA0105.1R	pNL1.3.CMV[secNluc/CMV]
pENTR-L1pLaclacZaL4	pRI101
pKO1	pFastBac1

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